General Cell Lines and Cell Cultures
Eurofins Discoverx ChemiScreen B2 Bradykinin Receptor Stable Cell Line, 2 vials. (HTS041C)
The ChemiScreen B2 Bradykinin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. bradykinin) based activation of BDKRB2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses BDKRB2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of BDKRB2, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen NOP/ORL1 Opioid Receptor Membrane Preparation, 1 vial. (HTS040M)
The ChemiScreen NOP/ORL1 Opioid Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of OPRL1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen M5 Muscarinic Acetylcholine Receptor Membrane Preparation, 1 vial. (HTS075M)
The ChemiScreen M5 Muscarinic Acetylcholine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CHRM5 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx Ready-to-Assay CCR2B Chemokine Receptor Frozen Cells, 2 Vials of Cells. (HTS007RTA)
The Ready-to-Assay CCR2B Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. eotaxin) based activation of CCR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CCR2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CCR2, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay GLP-2 Receptor Frozen Cells, 2 Vials of Cells. (HTS164RTA)
The Ready-to-Assay GLP-2 Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. GLP-2) based activation of GLP2R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GLP2R alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of GLP2R, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay 5-HT4B Serotonin Receptor Frozen Cells, 2 Vials of Cells. (HTS110RTA)
The Ready-to-Assay 5-HT4B Serotonin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. serotonin/5-HT) based activation of HTR4B GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses HTR4B alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of HTR4B, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen CysLT1 Leukotriene Receptor Stable Cell Line, 2 vials. (HTS061C)
The ChemiScreen CysLT1 Leukotriene Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. leukotriene) based activation of CysLT1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CysLT1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CysLT1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay V2 Vasopressin Receptor Frozen Cells, 2 Vials of Cells. (HTS060RTA)
The Ready-to-Assay V2 Vasopressin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. vasopressin) based activation of AVPR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses AVPR2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of AVPR2, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
ATCC Candida albicans; CATW 4/19
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
Eurofins Discoverx ChemiScreen EP1 Prostanoid Receptor Stable Cell Line, 2 vials. (HTS099C)
The ChemiScreen EP1 Prostanoid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin E2) based activation of PTGER1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of PTGER1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
